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CytoJournal 2005,  2:15

Efficiency of an inexpensive liquid-based cytology performed by cytocentrifugations: a comparative study using the histology as reference standard

1 Department of Pathology, CHU de Charleroi (Université Libre de Bruxelles), 1 Boulevard Zoé Drion, 6000 Charleroi, Belgium
2 Department of Pathology, CHU de Charleroi (Université Libre de Bruxelles), 1 Boulevard Zoé Drion, 6000 Charleroi; Laboratory of Molecular Virology, Pasteur Institute, 642 rue Engeland, 1180 Brussels, Belgium

Correspondence Address:
Christian Garbar
Department of Pathology, CHU de Charleroi (Université Libre de Bruxelles), 1 Boulevard Zoé Drion, 6000 Charleroi
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Source of Support: None, Conflict of Interest: None

PMID: 16164754

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Background :Although liquid-based cytology (LBC) is now recommended for cervical cancer screening, it requires expensive automated devices and materials. To evaluate the efficiency of inexpensive LBC methods relying on an inexpensive fixative liquid, Easyfix® , we compared the results obtained by the liquid-based cytology (LBC) diagnoses performed by cytocentrifugations (Papspin® and Turbitec® ) with those obtained by histology. Furthermore, we evaluated the efficiency of the fixative liquid, Easyfix® , to preserve HPV DNA in the collected samples. Method :266 LBC were compared with 174 colposcopies and 91 Loop Electrosurgical Excision Procedure (LEEP). Among the LBC, 51 were performed using the Papspin® system and 215 were performed using the Turbitec® system. To control the quality of the preservation liquid, Easyfix® , we correlated the results of HCII assays with those of HPV PCR. Results :For Papspin® and Turbitec® systems, the sensitivities were respectively 82.6% (95% CI: 61.2-95.0%, p < 0.001) and 75.0% (95% CI: 64.4-89.8%, p < 0.001) and the specificities were 92.6% (95%CI: 76.5-99.1%, p < 0.001) and 96.2% (95% CI: 91.3-98.7%, p < 0.001). We find no statistical difference between the results of the both systems (p = ns). The sensitivity of the HCII was 86.4% (95% IC: 77.4-92.8%, p < 0.001) and the specificity was 39.4% (95% CI: 31.2-48.1%, p < 0.001). The comparison between HCII and HPV-PCR shows a good correlation: the kappa was 0.89. Conclusion :LBC performed by cytocentrifugations are inexpensive, reduce inadequate smears, show excellent efficiency and allow HPV detection by molecular biology.


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