Prevalence and significance of psammoma bodies in cervicovaginal smears in a cervical cancer screening program with emphasis on a case of primary bilateral ovarian psammocarcinoma
Teresa Pusiol1, Anna M Parolari2, Irene Piscioli3, Luca Morelli1, Franca Del Nonno4, Stefano Licci4
1 Department of Pathology, "S. Maria del Carmine" Hospital, Rovereto (TN), Italy
2 Department of Gynecology, New Hospital of Arco (TN), Italy
3 Department of Radiology, Civil Hospital of Budrio (BO), Italy
4 Department of Pathology, "National Institute for Infectious Diseases – L. Spallanzani" IRCCS, Rome, Italy
|Date of Submission||18-Dec-2007|
|Date of Acceptance||16-Apr-2008|
|Date of Web Publication||16-Apr-2008|
Department of Pathology, "National Institute for Infectious Diseases – L. Spallanzani" IRCCS, Rome
Source of Support: None, Conflict of Interest: None
Background: The purpose of our study was to determine the prevalence and significance of psammoma bodies (PBs) in the cervicovaginal smears of the screening population of Trento district (Italy), with the description of the cytological presentation of an asymptomatic bilateral ovarian psammocarcinoma.
Methods: From 1993 to 2006, women with PBs detected on consecutively screened cervical smears were identified from the computerized pathology database of Rovereto Hospital. The follow-up period was set from the time of cytological diagnosis to May 31 st , 2007. Clinical information was obtained from retrospective review of women's medical records. The source of PBs was identified with adequate diagnostic procedures.
Results: PBs were found in six of the 201,231 Papanicolaou screening smears (0.0029%). Benign conditions (intrauterine device, inclusion ovarian cysts and ovarian cystoadenofibroma with PBs) were found in four patients. In two cases, PBs were associated with malignant cells; a bilateral ovarian malignancy was diagnosed in both cases, a serous adenocarcinoma and a psammocarcinoma.
Conclusion: PBs in the cervicovaginal smears are a rare finding, associated more often with benign conditions than with malignancies. Moreover, to our knowledge, our case of primary ovarian psammocarcinoma is the first report in which the presence of malignant cells and PBs in the cervicovaginal and endometrial smears represents the first manifestation of disease.
|How to cite this article:|
Pusiol T, Parolari AM, Piscioli I, Morelli L, Nonno FD, Licci S. Prevalence and significance of psammoma bodies in cervicovaginal smears in a cervical cancer screening program with emphasis on a case of primary bilateral ovarian psammocarcinoma. CytoJournal 2008;5:7
|How to cite this URL:|
Pusiol T, Parolari AM, Piscioli I, Morelli L, Nonno FD, Licci S. Prevalence and significance of psammoma bodies in cervicovaginal smears in a cervical cancer screening program with emphasis on a case of primary bilateral ovarian psammocarcinoma. CytoJournal [serial online] 2008 [cited 2019 Sep 17];5:7. Available from: http://www.cytojournal.com/text.asp?2008/5/1/7/41199
| » Background|| |
The presence of psammoma bodies (PBs) in cervicovaginal smears (CVS) is a rare finding, the prevalence ranging from 0.00047% to 0.057% in large series studies [1-6]. PBs on Papanicolau smears have been described in association with a wide variety of gynaecological conditions including malignant tumors, such as malignant serous epithelial ovarian tumors , endometrial carcinoma with PBs , neuroendocrine carcinoma of the cervix , primary peritoneal borderline tumour , tubal serous carcinoma and cervical clear cell carcinoma . Exceptionally, the primary site of origin remains unclear [1,4]. However, they are most frequently associated with benign conditions, presumably related to the use of an intrauterine device (IUD) [11-13], to a history of oral contraception  or associated with a benign ovarian neoplasm , inflammatory lesions, such as tubo-ovarian adhesions and endosalpingiosis [16,17], endometrial benign hyperplasia and endometrial polyps . The most reliable predictor of a malignancy is the association of malignant cells with PBs on the smears. The purpose of our work is to determine the prevalence and significance of PBs in a sequential series of CVS in the screening population of Trento district (Italy). Furthermore, we illustrate the first case report, as far as we are aware, of a bilateral primary ovarian psammocarcinoma (PC), in which PBs found in association with malignant cells in the cervicovaginal and endometrial smears represent the first manifestation of disease.
| » Methods|| |
We identified from the computerized pathology database of Rovereto Hospital all cases describing the presence of PBs in CVS, with a retrospective review of 201,231 screening cytological exams from 1993 to 2006. The cervical cancer screening program was performed according to Walton report  and cytological diagnoses were reported according to the Bethesda System and its modifications in the years [19,20]. The diagnostic connotations were recorded using standardized Systematized Nomenclature of Medicine coding (PBs: M-55580). Clinical informations were obtained from retrospective review of women's medical records before and after identification of PBs (age, gravity, parity, menopausal status, symptoms present at the time of abnormal papanicolau smear, contraceptive and gynaecological history). Follow up informations were collected from the time of cytological diagnosis to May 31 st , 2007. Adequate diagnostic procedures (endometrial aspiration, hysteroscopy, uterine curettage, vaginal and abdominal ecography, TC) were performed in order to identify the source of PBs.
| » Results and Discussion|| |
In the retrospective review of 201,231 screening program cytology reports we identified 6 Papanicolaou smears with PBs (0.0029%).
Four of them belonged to premenopausal women and PBs were found in absence of atypical cells [Figure 1a]. Endometrial aspiration was negative either for PBs or for malignant cells.
Two of these patients (40 years old, gravida 2 para 2 and 36 years old, gravida 1 para 1) had an IUD. Because of a possible coexistence of a gynaecologic malignancy the surgeons performed a laparoscopy with removal of the IUD. The abdominal and pelvic organs, including the ovaries, revealed no abnormalities.
The third patient (46 years old, gravida 1 para 2) showed multiple, small cysts in the right ovary. A laparoscopic resection was performed and histological examination revealed benign inclusion ovarian cysts with intraparietal PBs.
In the fourth patient (45 years old, gravida 1, para 1), a pelvic ultrasonography showed a cystic structure, 1.5 cm in diameter, in the right ovary. For not specified circumstances, an abdominal hysterectomy with bilateral salpingo-oophorectomy and appendicectomy was performed. Histopathologic examination revealed a serous cystoadenofibroma with PBs of the right ovary [Figure 1b]. The left ovary had no significant lesions.
Papanicolau smears were repeated three to six months after the initial identification of PBs in the four patients, and none of them showed persistent PBs in the smears, even after a follow up period of 3-8 years.
The last two patients with PBs on the smears had a bilateral ovarian malignancy with a diffuse involvement of the abdominal cavity. Rare blue-stained, laminated, calcified bodies, identified as PBs were found in association with diagnostic malignant cells, which were variable in size and shape and had large nuclei with prominent nucleoli [Figure 2a]. Both endometrial aspirations contained several PBs isolated or surrounded by clusters of columnar cells showing a moderate degree of atypia [Figure 2b]. Rare papillary groups of atypical glandular cells were present. Both patients underwent an exploratory laparotomy, with a subsequent total abdominal hysterectomy, bilateral salpingo-oophorectomy, omentectomy, regional lymphadenectomy and multiple peritoneal biopsies.
In one patient (56-years-old, gravida 2, para 2) a preoperative vaginal ultrasonography revealed a dishomogeneous uterine corpus, 7,3 × 5,4 cm in size, with multiple nodular lesions referable to leiomyomas located in the anterior wall of the myometrium. Intraoperatory findings showed a right ovarian mass, white and firm, 4 cm in diameter and another nodular lesion on the surface of the left ovary, grey-white, well circumscribed and 2 cm in diameter. The invasive thickness of the ovarian stroma was 1,2 cm on the cut surface. The omentum and peritoneal surface were involved by multiple tumor nodules (2 cm in maximum diameter). Intraoperative histological examination was consistent with the diagnosis of bilateral moderately differentiated ovarian serous adenocarcinoma. The final pathologic report confirmed the diagnosis made on frozen sections. Additonal feature was the presence of multiple psammomatous calcifications [Figure 3a]. Neoplastic infiltration was observed in the endometrium, without myometrium invasion. The omental nodules showed the features of invasive implants. Malignant cells and PBs were found in the peritoneal washing. Twenty-seven regional lymph nodes were free of malignancy. She was placed in the International Federation of Gynecology and Obstetrics (FIGO) stage III B. The patient received chemotherapy. Since then, she has been followed up with several CT scans of abdomen and pelvis and has recovered without incident.
In the other patient (50 years-old, gravida 1, para 1) exploratory laparotomy showed a diffuse white-grey nodularity on the surface of both ovaries. Two omental and three peritoneal nodules (2 cm in maximum diameter) were found. Intraoperative histological examination was consistent with the diagnosis of bilateral ovarian serous carcinoma with extensive psammoma bodies formation, showing the typical proliferative pattern of PC, according to the diagnostic criteria defined by Gilks , and infiltrating the ovarian stroma (invasive tickness > 1 cm) [Figure 3b] . The nodules removed from the peritoneal and omental surfaces were confirmed to be neoplastic involvement [Figure 3c]. The final histological diagnosis was primary bilateral psammocarcinoma of the ovaries with macroscopic omental and peritoneal metastases, invasion of both tubaric lumens [Figure 3d], extension to endometrium without myometrium infiltration. The eighteen lymph nodes sampled were free of disease. She was placed in the FIGO stage III B. Following surgery no gross tumor was remaining. The peritoneal cytology was positive. The patient was started on chemotherapy. After 10.5 years of follow up the patient is alive, free of disease.
The prevalence of PBs in our series of consecutively screened Papanicolau smears is 6 per 201,231 (0.0029%), which confirms that this is a very unusual finding, in agreement with other large series of CVS previously reported in the literature and summarized in [Table 1]. The actual prevalence and significance of PBs in CVS in a cervical cancer screening program, based on the Walton report , have not been previously reported. The Walton report, a document of the Canadian task force on cervical cancer screening, recommended a program based on chronologic age, calling for 3- and 5-year intervals between examinations. The six cases described in our study exhibit different clinical diagnoses, with a higher prevalence of benign lesions associated with PBs, according to the literature data.
The observation of PBs on CVS should not be ignored and should alert the clinician in order to evaluate the possibility of a coexistent carcinoma, even though a review of the literature reveals that in more than 50% of patients PBs are associated with benign conditions [1-6]. All benign cases are described in relatively young individuals, the oldest being 46 years old. In two of our cases the presence of IUD was considered the source of PBs on the basis of similar, well documented reported cases [11-13]. Another source of PBs in cervicovaginal smears are benign inclusion cysts , like in another case of ours. The rupture of an ovarian cyst, followed by aspiration of its contents and of minute parietal fragments with PBs by the Fallopian tube More Details is the most likely explanation for the cytological finding. Calcifications and PBs are present in almost the 30% of ovarian cystoadenofibromas  and this can justify the presence of PBs in CVS, like in the fourth benign case of ours; the same mechanism above described may be evoked.
When PBs in the CVS are associated with malignant or borderline tumors, the ovary is the most common primary site (51%), serous cystoadenocarcinoma being the most common neoplasm, followed by the uterine corpus (30%) . In our two cases, the presence of malignant cells with PBs in the lumen of the fallopian tubes may be the mechanism responsible for the cytological findings. In one of them, the report was related to an unknown primary bilateral ovarian PC. This represents a rare invasive variant of serous carcinoma arising in the ovary and in the peritoneum [21,25-31].
In the first description by Gilks et al. , eight cases were primary ovarian neoplasms and three were arising from omentum. These authors reported the accurate strict histological diagnostic criteria for the diagnosis of PC, according to which psammoma bodies must replace at least 75% of the papillae, cytological features must be low grade and the neoplastic epithelium must be arranged in small nests with no areas of solid proliferation. Potential mechanisms responsible for the characteristic, extensive PBs formation include the accumulation of successive layers of calcium on single necrotic or degenerated tumor cells .
Primary peritoneal PC is a very unusual variant of extraovarian neoplasms that histologically resemble surface-epithelial-stromal tumours of ovarian origin.
In these cases, an adequate sampling of the omentum, the adnexa, and the uterine serosa, as well as of any dominant mass, is required to establish the primary origin of the malignancy.
In our case, a stromal invasion with tumor size more than 5 × 5 mm in diameter and the extensive involvement of the ovary surface have been considered the conclusive criteria of primary bilateral ovarian PC.
Clinical behaviour of PCs closely resembles that of borderline serous tumours with a protracted course and a favourable prognosis. Nevertheless, tumors with an aggressive course have been described in the literature [30,31]. In the case reported by Poggi et al.  no adjuvant therapy was proposed because of the supposed indolent behaviour, with a recurrence of disease after eighteen months. In the second case described by Akbulut et al. , 9 cycles of chemotherapy were performed, after a complete debulking, with a recurrence of the disease after 5 years and metastatic infiltration of the vertebral corpus one year later, in spite of other 6 cycles of chemotherapy.
Our patient chose to receive adjuvant chemotherapy and is alive and free from disease after 10.5 years of follow-up. The excellent prognosis is in agreement with previous reports.
| » Conclusion|| |
Our study performed on a large screening series confirms that the presence of PBs in CVS is a rare finding, more often associated with benign conditions. Anyway, this finding should never be underestimated and a careful examination of the morphological features of the cell groups is crucial to establish the source of PBs and disclose an underlying malignancy. Additional clinical investigations are always recommendable.
Moreover, we have described the first report of ovarian PC in which the presence of malignant cells and PBs in the cervicovaginal and endometrial smears represents the first manifestation of the disease.
| » Competing interests|| |
The authors declare that they have no competing interests.
| » Authors' contributions|| |
TP, AMP, IP and LM participated equally in the design of the paper and in the collection of the clinico-pathological data. FDN and SL participated in the revaluation of the cytological and histological specimens and in the drafting of the manuscript. SL revised critically the final version of the manuscript. All authors read and approved the final manuscript.
| » Acknowledgements|| |
Written consent was obtained from the patient or their relatives for publication of the study.
| » References|| |
|1.||Nicklin JL, Perrin L, Obermair A, McConachie I, Cominos D: The significance of psammoma bodies on cervical cytology smears. Gynecol Oncol 2001, 83: 6-9. |
|2.||Parkash V, Chacho MS: Psammoma bodies in cervicovaginal smears: incidence and significance. Diagn Cytopathol 2002, 26: 81-86. |
|3.||Muntz HG, Goff BA, McGonigle K, Isacson C: The significance of psammoma bodies in screening cervical cytologic smears. Am J Obstet Gynecol 2003, 188: 1609-1614. |
|4.||Kern SB: Prevalence of psammoma bodies in Papanicolaou-stained cervicovaginal smears. Acta Cytol 1982, 2: 571-613. |
|5.||De Peralta-Venturina M, Kini SR: Clinical significance of psammoma bodies in cervicovaginal smears (CVS): a 5-year experience. Lab Invest 2000, 80: 32A1. |
|6.||Zreik TG, Rutherford TJ: Psammoma bodies in cervicovaginal smears. Obstet Gynecol 2001, 97: 693-695. |
|7.||Beyer-Boon ME: Psammoma bodies in cervicovaginal smears: an indicator of the presence of ovarian carcinoma. Acta Cytol 1974, 18: 41-44. |
|8.||Weaver RJ, Wilson R: Endometrial carcinoma with psammoma bodies in the vaginal smear. Am J Obstet Gynecol 1967, 97: 869-870. |
|9.||Russin VL, Valente PT, Hanjani P: Psammoma bodies in neuroendocrine carcinoma of the uterine cervix. Acta Cytol 1987, 31: 791-795. |
|10.||Bell DA, Scully RE: Serous borderline tumors of the peritoneum. Am J Surg Pathol 1990, 14: 230-239. |
|11.||Highman DR, Magrane DM: Calcified bodies and the intrauterine device. Acta Cytol 1971, 15: 473-475. |
|12.||Boon ME, Kirk RS, De Graaff Guilloud JC: Psammoma bodies and some opportunistic infections detected in cervical smears of women fitted with an IUD. Contracept Deliv Syst 1981, 2: 231-236. |
|13.||Barter JF, Orr JW, Holloway RW, Hatch KD, Shingelton HM: Psammoma bodies in a cervicovaginal smear associated with an intrauterine device. A case report. J Reprod Med 1987, 32: 147-148. |
|14.||Valicenti JF, Priester SK: Psammoma bodies of benign endometrial origin in cervicovaginal cytology. Acta Cytol 1977, 21: 550-552. |
|15.||Kanbour A, Doshi N: Psammoma bodies and detached ciliary tufts in a cervicovaginal smear associated with benign ovarian cystoadenofibroma. Acta Cytol 1980, 24: 549-552. |
|16.||Hallman KB, Nahhas WA, Connelly PJ: Endosalpingiosis as a source of psammoma bodies in a Papanicolaou smear. A case report. J Reprod Med 1991, 36 (9) : 675-678. |
|17.||Seguin RE, Ingram K: Cervicovaginal psammoma bodies in endosalpingiosis. A case report. J Reprod Med 2000, 45 (6) : 526-528. |
|18.||Walton RJ, (task force chmn): Cervical cancer screening programs [DNH&W report]. Can Med Assoc J 1976, 114: 1003-1033. |
|19.||National Cancer Institute Workshop: The 1988 Bethesda System for reporting cervical/vaginal cytologic diagnoses. JAMA 1989, 262: 931-934. |
|20.||Solomon D, Davey D, Kurman R, Moriarty A, O'Connor D, Prey M, Raab S, Sherman M, Wilbur D, Wright T, Young N, Forum Group Members; Bethesda 2001 Workshop: The 2001 Bethesda System: terminology for reporting results of cervical cytology. JAMA 2002, 287: 2114-2119. |
|21.||Gilks CB, Bell DA, Scully RE: Serous psammocarcinoma of the ovary and peritoneum. Int J Gynecol Pathol 1990, 9: 110-121. |
|22.||Luzzatto R, Brucker N: Benign inclusion cysts of the ovary associated with psammoma bodies in vaginal smears. Acta Cytol 1981, 25: 282-284. |
|23.||Czernobilsky B, Borenstein R, Lancet M: Cystadenofibroma of the ovary. Cancer 1974, 34: 1971-1974. |
|24.||Fadare O, Chacho MS, Parkash V: Psammoma bodies in cervicovaginal smears: significance and practical implications for diagnostic cytopathology. Adv Anat Pathol 2004, 11: 250-261. |
|25.||Pakos E, Funke A, Tschubel K, Pfeifer U: Serous psammocarcinoma of the ovary. Case report and literature review. Pathologe 1997, 18: 463-466. |
|26.||Powell JL, McDonald TJ, White WC: Serous psammocarcinoma of the ovary. South Med J 1998, 91: 477-480. |
|27.||Cobellis L, Pezzani I, Castaldi P, Bome A, Santopietro R, Petraglia F: Ovarian psammocarcinoma with peritoneal implants. Eur J Obstet Gynecol Reprod Biol 2003, 107: 217-219. |
|28.||Kelley JL, Capelle SC, Kanbour-Shakir A: Serous psammocarcinoma of the ovary in an adolescent female. Gynecol Oncol 1995, 59: 309-311. |
|29.||Rettenmaier MA, Goldstein BH, Epstein HD, Brown JV, Micha JP: Serous psammocarcinoma of the ovary: an unusual finding. Gynecol Oncol 2005, 99: 510-511. |
|30.||Poggi SH, Bristow RH, Nieberg RK, Berek JS: Psammocarcinoma with an aggressive course. Obstet Gynecol 1998, 92: 659-961. |
|31.||Akbulut M, Kelten C, Bir F, Soysal ME, Duzcan SE: Primary peritoneal serous psammocarcinoma with recurrent disease and metastasis: a case report and review of the literature. Gynecol Oncol 2007, 105 (1) : 248-251. |
|32.||Johannessen JV, Sobrinho-Simoes M: The origin and significance of thyroid psammoma bodies. Lab Invest 1980, 43: 287-296. |
[Figure 1a], [Figure 1b], [Figure 2a], [Figure 2b], [Figure 3a], [Figure 3b], [Figure 3c], [Figure 3d]
|This article has been cited by|
||Calcareous concretions and psammoma bodies in sputum smears
| ||Rafael Martínez-Girón,Santiago Martínez-Torre,María Luisa Tamargo-Peláez,María Dolores López-Cabanilles,Concepción Torre-Bayón |
| ||Diagnostic Cytopathology. 2014; : n/a |
|[Pubmed] | [DOI]|
||Psammocarcinome séreux agressif de l’ovaire : à propos d’un cas et revue de la littérature
| ||M. Oukabli, K. Andaloussi, A. Boudhas, N. Harchichi, A. Albouzidi |
| ||Journal africain du cancer / African Journal of Cancer. 2012; |
|[VIEW] | [DOI]|
|| A bilateral ovarian psammomatous papillary cystic adenocarcinoma in a German Shepherd bitch
| ||Petterino, C., Modesto, P., Ratto, A. |
| ||Comparative Clinical Pathology. 2010; 19(4): 389-395 |
|| Are the presence of psammoma bodies in cervicovaginal smear an indicator of ovarian carcinoma?: Case report | [Servikovaginal smeardeki psammoma cisimcikleri ovaryan kanser varliǧinin göstergesi midir?]
| ||Ergin, N., Api, M., Çilesiz, P., Yayla, M., Çetin, A. |
| ||Turkiye Klinikleri Jinekoloji Obstetrik. 2010; 20(3): 188-191 |
||Psammoma bodies in cervical smear in association with keratinizing squamous cell carcinoma of cervix: A case report
| ||Pillai, K.R. and Sujathan, K. and Mani, KS and Jayalal, KS and Somanathan, T. |
| ||Diagnostic Cytopathology. 2009; 37(6): 450-454 |
||A unique case of ovarian psammocarcinoma with mediastinal, pulmonary, subcutaneous, and omental metastases
| ||Chase, D.M., Sparks, D.A., Gener, M., Smith, J. |
| ||Archives of Gynecology and Obstetrics. 2009; 280(2): 283-286 |
||A unique case of ovarian psammocarcinoma with mediastinal, pulmonary, subcutaneous, and omental metastases
| ||Daniel M. Chase,Dorothy A. Sparks,Melissa Gener,James Smith |
| ||Archives of Gynecology and Obstetrics. 2009; 280(2): 283 |
|[Pubmed] | [DOI]|
|| CytoJournal′s move to the new platform: More on financial model to the support open-access charter in cytopathology, publication quality indicators, and other issues
| ||Shidham, V., Pitman, M., Demay, R., Atkinson, B. |
| ||CytoJournal. 2008; 5(art 15) |