Fine needle aspiration diagnosis of Rosai-Dorfman Disease in an osteolytic lesion of bone
Shaoying Li, Zhijie Yan, Nirag Jhala, Darshana Jhala
Department of Pathology, University of Alabama at Birmingham, Birmingham, AL, USA
|Date of Submission||19-Jan-2010|
|Date of Acceptance||24-Apr-2010|
|Date of Web Publication||02-Jul-2010|
Department of Pathology, University of Alabama at Birmingham, Birmingham, AL
Source of Support: None, Conflict of Interest: None
Sinus histiocytosis with massive lymphadenopathy (SHML) or Rosai-Dorfman disease (RDD) is an uncommon but well-defined benign self-limited clinicopathological entity. It mainly involves lymph nodes. Extranodal involvement is seen in up to 43% of cases, with the most common location in the head and neck region. Primary RDD occurring in the bone is rare with only twelve cases reported in the literature to date, all diagnosed on histology except one by fine needle aspiration (FNA) cytology. We report a case of RDD diagnosed by FNA cytology in a 28 year-old female presented as an osteolytic lesion of superior pubic ramus where the differential diagnosis included a sarcoma and lymphoma. Based on the cytologic findings, a diagnosis of a RDD was considered during the rapid FNA on site with no clinical history provided. The diagnosis of RDD was further confirmed by immunohistochemical stains and histology diagnosis. Our findings show that even in the absence of a clinical history, FNA is a less invasive and a very reliable tool for the diagnosis of SHML (RDD).
Keywords: Bone, Rosai-Dorfman disease, FNA
|How to cite this article:|
Li S, Yan Z, Jhala N, Jhala D. Fine needle aspiration diagnosis of Rosai-Dorfman Disease in an osteolytic lesion of bone. CytoJournal 2010;7:12
|How to cite this URL:|
Li S, Yan Z, Jhala N, Jhala D. Fine needle aspiration diagnosis of Rosai-Dorfman Disease in an osteolytic lesion of bone. CytoJournal [serial online] 2010 [cited 2018 May 25];7:12. Available from: http://www.cytojournal.com/text.asp?2010/7/1/12/65058
| » Introduction|| |
Sinus histiocytosis with massive lymphadenopathy (SHML) or Rosai-Dorfman Disease More Details (RDD) is a rare but well-defined benign self-limited clinicopathological entity. It commonly involves lymph nodes. Extranodal involvement is seen in up to 43% of cases, the most common location is the head and neck region (75%), where the skin, orbit, nasal cavity and paranasal sinuses are the most common sites. [1,2] Primary RDD occurring in the bone is rare with only 12 cases reported in the literature to date, all diagnosed on histology except one by FNA cytology .  FNA is a safe, relatively non-invasive diagnostic procedure often used to diagnose neoplasm and inflammatory disease. The characteristic cytomorphological features of RDD obtained via FNA have been described in cases reports. [4-8] We report a case of RDD diagnosed by FNA cytology in a 28 year-old female presented with an osteolytic lesion of the superior pubic ramus.
| » Case Report|| |
A 28 - year-old woman presented with progressive hip girdle pain for several months and underwent imaging studies which revealed a destructive lytic bone lesion around the right acetabulum and proximal femur involving the superior pubic ramus. The pain was worsening with weight bearing and was controlled fairly well with pain medications. The patient had no significant constitutional symptoms. She denied any weight loss. Review of systems was unremarkable. Her past medical history was also unremarkable.
The patient's laboratory values at the time of admission included hemoglobin of 12.2 g/dl; hematocrit of 35.3%; white blood cell count of 5,630/mm3; platelet count of 244,000 mm3 and erythrocyte sedimentation rate of 50 mm/hr.
An anterior-posterior pelvis radiograph showed an osteolytic bone lesion in the right superior pubic ramus and acetabulum [Figure 1]. A computer tomography (CT) scan revealed the same destructive lytic bone lesion around the right acetabulum and superior pubic ramus. There was marked destruction of the cortex and an associated soft tissue mass extending into the obturator internus muscle. There was no lymphadenopathy. Differential diagnosis included metastatic neoplasms versus primary bone tumors or lymphoma.
A CT guided FNA of the right superior pubic ramus was performed using a 22 gauge needle. Air-dried smears were stained with Diff-Quik (Baxter, Melrow Park, IL, USA). The needles from each pass along with remaining FNA samples were rinsed with Hank's solution and used for making cell block. Rapid interpretation was given based on the finding noted on the Diff-Quik stain. The specimen was hypocellular and revealed varying numbers of large histiocytes with prominent emperipolesis. Those large histiocytes contain numerous phagocytosed intact lymphocytes, neutrophils and rare plasma cells within the cytoplasm [Figure 2]a. Fine vacuoles in the cytoplasm of the histiocytes were easily appreciated. There were polymorphous lymphoid elements and relative lack of eosinophils; with slightly increased numbers of plasma cells in the background [Figure 2]b. No pleomorphic spindle cells or monomorphic lymphoid cells were noted, excluding the possibility of sarcoma or lymphoma. Based on these findings, a diagnosis of a RDD was considered. No clinical history was provided at the time of rapid interpretation.
Immunoperoxidase stains for antibodies to S - 100 protein and CD1a were performed on the cell block. The histiocytes were intensely positive for S - 100 protein [Figure 2]c and negative for CD1a, supporting the diagnosis of RDD.
A follow-up biopsy confirmed the diagnosis of RDD histologically. Due to progressive pain and concern for a potential future pathologic fracture, the patient was advised to undergo radiation therapy and chemotherapy that she was to pursue in a different city from where she lives. Follow- up had been lost since then.
| » Discussion|| |
SHML or RDD was first described by Rosai J. and Dorfman R.F. in 1969.  It is a rare but well-defined clinicopathological entity. It affects all age groups, but is predominantly seen in children and adolescents and more commonly seen in males. It is a histiocytic proliferative disorder of unknown etiology characterized clinically by painless bilateral cervical lymphadenopathy in the majority of patients; accompanied by fever, leukocytosis, elevated erythrocyte sedimentation rate (ESR), and hypergammaglobulinemia. [2,10] Extranodal involvement is seen in up to 43% of cases. The most common location is the head and neck region (75%), where the skin, orbit, nasal cavity and paranasal sinuses are the most common sites. [1,2] Primary RDD occurring in the bone is rare with only 12 cases reported in the literature to date, all diagnosed on histology except one by fine needle aspiration cytology.  Most cases of RDD follow a waxing and waning course over years with eventually complete regression and do not require any treatment; therefore, the correct diagnosis is very important. Histologically, severely dilated lymphatic sinuses are occupied by lymphocytes, plasma cells and numerous large histiocytes resulting in partial or complete architecture effacement. The hallmark of this disease is emperipolesis or lymphocytophagocytosis, that is, many large histiocytes contain numerous phagocytosed intact lymphocytes (sometimes other cells such as plasma cells and red blood cells) within the cytoplasm. It is a non-specific but diagnostic feature of RDD. Cytological findings demonstrate a high degree of histological correlation and consistency. The significant cytomorphological features of RDD reported include cellular smears containing large histiocytes (150/200 μm) with prominent emperipolesis, halos around each of the phagocytosed cells, fine vacuoles in the cytoplasm of the histiocytes containing large amount of neutral lipid demonstrated by Oil red O stain, relatively lack of follicular center cells and eosinophils, and an increased number of plasma cells in the background. [4-8, 11, 12] All these features were present in our case. We agree with most other reports that the cytological features of RDD are very characteristic so that FNA cytology is a reliable diagnostic modality. However, in spite of its well-defined cytomorphological features, diagnosis of SHML (RDD) may be very challenging, especially in extranodal sites. Because of its non-specific clinical presentation and sometimes with only subtle changes or even with atypical features as well as its rarity, it may be overlooked or misinterpreted by cytopathologists. Lymphophagocytosis can be seen in many different clinical settings, of note, sinus hyperplasia, and virus associated lymphophagocytic syndromes, lymphadenitis caused by Salmonella More Details, rhinoscleroma, and histoplasmosis. Hence the differential diagnosis of SHML (RDD) in general and in particular should include the above mentioned entities. For virus associated lymphophagocytic syndromes, except different clinical manifestation such as skin rash, cytologically viral cytopathic effect is very helpful for the differential diagnosis. Rhinoscleroma is a chronic granulomatous condition of the upper respiratory tract and the presence of granulomas is a distinctive feature. Histoplasmosis will have the tiny intracellular organisms present. Additional clinical, morphological, and laboratory information on these entities will be helpful in making a correct diagnosis. The differential diagnoses also include Langerhans' cell histiocytosis (LCH), malignant histiocytosis, and non-specific reactive histiocytosis.  LCH occurs mainly in children and young adults with a male predominance. It also causes lytic bone lesions. In LCH, the histiocytes have a longitudinal groove and a coffee-bean appearance. The inflammatory background consists of large numbers of eosinophils. Langerhans cells express CD1a and S - 100 but lack expression of CD45. RDD cells consistently express S - 100 protein and CD68, but negative for CD1a. CD1a is a more specific marker for LCH and an important feature to differentiate LCH with other histiocytic lesions like RDD. Ultrastructrally, the presence of cytoplasmic rod or tennis-racket like Birbeck granules are characteristic of Langerhans cells which are not present in histiocytes of RDD or other histiocytic lesions. Malignant histiocytosis is very rare and mainly involves extraskeletal site. The cells are large and round with abundant eosinophilic cytoplasm. Emperipolesis is not seen. The cells are positive for lysozyme, which is negative or very weak in RDD. Reactive histiocytosis is a benign entity mainly involving lymph nodes. The histiocytes in this benign entity are negative for S - 100 protein. In addition, emperipolesis could be overlooked or mistaken for tingible body macrophages as seen in follicular hyperplasia with plasmocytosis and sinus histiocytosis.  Extracaution has to be taken when interpreting lymph node or extranodal FNA cytology smears. Rarely SHML has been identified as an isolated phenomenon in the lymph nodes affected by non-Hodgkin's lymphoma (NHL) or Hodgkin's disease  and even NHL may follow SHML.  In a solitary lytic bone lesion as seen in our case, giant cell tumor of the bone or osteosarcoma has to be considered in the differential diagnosis. Emperipolesis may be misinterpreted as osteoclasts or giant cells. However, other cytomorphologic features and clinical information as well as immunohistochemical stains would be beneficial in making a correct diagnosis.
| » Conclusion|| |
Our findings show that even in the absence of a clinical history, FNA is a less invasive and a very reliable tool for the diagnosis of SHML (RDD).
| » Competing Interest Statement by all Authors|| |
No competing interest to declare by any of the authors.
| » Authorship Statement by all Authors|| |
Each author acknowledges that this final version was read and approved. All authors of this article declare that we qualify for authorship as defined by ICMJE http://www.icmje.org/#author . Each author has participated sufficiently in the work and take public responsibility for appropriate portions of the content of this article.
| » Ethics Statement by all Authors:|| |
As this is case report without identifiers, our institution does not require approval from Institutional Review Board (IRB) (or its equivalent).
| » References|| |
|1.||Foucar E, Rosai J, Dorfman R. Sinus histiocytosis with massive lymphadenopathy (Rosai-Dorfman disease): review of the entity. Semin Diagn Pathol 1990;7:19-73. |
|2.||Gaitonde S. Multifocal, extranodal sinus histiocytosis with massive lymphadenopathy: an overview. Arch Pathol Lab Med 2007;131:1117-21. |
|3.||Goel MM, Agarwal PK, Agarwal S. Primary Rosai-Dorfman disease of bone without lymphadenopathy diagnosed by fine needle aspiration cytology. A case report. Acta Cytol 2003;47:1119-22. |
|4.||Das DK, Gulati A, Bhatt NC, Sethi GR. Sinus histiocytosis with massive lymphadenopathy (Rosai-Dorfman disease): report of two cases with fine-needle aspiration cytology. Diagn Cytopathol 2001;24:42-5. |
|5.||Deshpande V, Verma K. Fine needle aspiration (FNA) cytology of Rosai Dorfman disease. Cytopathology 1998;9:329-35. |
|6.||Hummel P, Waisman J, Chhieng D, Yan Z, Cohen JM, Cangiarella J. Fine-needle aspiration cytology of Rosai-Dorfman disease of the breast: A case report. Diagn Cytopathol 1999;21:287-91. |
|7.||Panikar N, Agarwal S. Salivary gland manifestations of sinus histiocytosis with massive lymphadenopathy: fine-needle aspiration cytology findings. A case report. Diagn Cytopathol 2005;33:187-90. |
|8.||Pettinato G, Manivel JC, d'Amore ES, Petrella G. Fine needle aspiration cytology and immunocytochemical characterization of the histiocytes in sinus histiocytosis with massive lymphadenopathy (Rosai-Dorfman syndrome). Acta Cytol 1990;34:771-7. |
|9.||Rosai J, Dorfman RF. Sinus histiocytosis with massive lymphadenopathy. A newly recognized benign clinicopathological entity. Arch Pathol 1969;87:63-70. |
|10.||Rosai J, Dorfman RF. Sinus histiocytosis with massive lymphadenopathy: a pseudolymphomatous benign disorder. Analysis of 34 cases. Cancer 1972;30:1174-88. |
|11.||Pιrez-Guillermo M, Sola-Pιrez J, Rodrνguez-Bermejo M. Malacoplakia and Rosai-Dorfman disease: two entities of histiocytic origin infrequently localized in the female breast--the cytologic aspect in aspirates obtained via fine-needle aspiration cytology. Diagn Cytopathol 1993;9:698-704. |
|12.||Schmitt FC. Sinus histiocytosis with massive lymphadenopathy (Rosai-Dorfman disease): cytomorphologic analysis on fine-needle aspirates. Diagn Cytopathol 1992;8:596-9. |
|13.||Jaffe R WL, Facchetti F, Grogan TM, Pileri SA, Chan JKC, Fletcher CD. Histiocytic and Dendritic Cell Neoplasms. In: Swerdlow SH, Harris NL, Jaffe ES, Pileri SA, Stein H, Thiele J, et al. editors. WHO Classification of Tumors of Haematopoitic and Lymphoid Tissues. 4 th ed. Lyon: IARC; 2008. p. 356-60. |
|14.||Alvarez Alegret R, Martinez Tello A, Ramirez T, Gαllego P, Martinez D, Garcνa Julνan G. Sinus histiocytosis with massive lymphadenopathy (Rosai-Dorfman disease): diagnosis with fine-needle aspiration in a case with nodal and nasal involvement. Diagn Cytopathol 1995;13:333-5. |
|15.||Maia DM, Dorfman RF. Focal changes of sinus histiocytosis with massive lymphadenopathy (Rosai-Dorfman disease) associated with nodular lymphocyte predominant Hodgkin's disease. Hum Pathol 1995;26:1378-82. |
|16.||Koduru PR, Susin M, Kolitz JE, Soni M, Teichberg S, Siques MJ, et al. Morphological, ultrastructural, and genetic characterization of an unusual T-cell lymphoma in a patient with sinus histiocytosis with massive lymphadenopathy. Am J Hematol 1995;48:192-200. |
[Figure 1], [Figure 2]
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