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 REVIEW
CytoJournal 2010,  7:3

Integrating a FISH imaging system into the cytology laboratory


1 ARUP Institute for Clinical and Experimental Pathology®, Associated Regional and University Pathologists (ARUP) Laboratories, Inc., Salt Lake City, Utah, USA
2 Department of Cytology, Associated Regional and University Pathologists (ARUP) Laboratories, Inc., Salt Lake City, Utah, USA
3 Technical Operations, Associated Regional and University Pathologists (ARUP) Laboratories, Inc., Salt Lake City, Utah, USA
4 ARUP Institute for Clinical and Experimental Pathology® and Department of Pathology, Associated Regional and University Pathologists (ARUP) Laboratories, Inc., Salt Lake City, Utah; Laboratory Medicine Consultants, Ltd., Las Vegas, NV, USA

Correspondence Address:
Joel S Bentz
ARUP Institute for Clinical and Experimental Pathology® and Department of Pathology, Associated Regional and University Pathologists (ARUP) Laboratories, Inc., Salt Lake City, Utah; Laboratory Medicine Consultants, Ltd., Las Vegas, NV
USA
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1742-6413.62258

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We have implemented an interactive imaging system for the interpretation of UroVysion fluorescence in situ hybridization (FISH) to improve throughput, productivity, quality control and diagnostic accuracy. We describe the Duet imaging system, our experiences with implementation, and outline the financial investment, space requirements, information technology needs, validation, and training of cytotechnologists needed to integrate such a system into a cytology laboratory. Before purchasing the imaging system, we evaluated and validated the instrument at our facility. Implementation required slide preparation changes, IT modifications, development of training programs, and revision of job descriptions for cytotechnologists. A darkened room was built to house the automated scanning station and microscope, as well as two imaging stations. IT changes included generation of storage for archival images on the LAN, addition of external hard drives for back-up, and changes to cable connections for communication between remote locations. Training programs for cytotechnologists, and pathologists/fellows/residents were developed, and cytotechnologists were integrated into multiple steps of the process. The imaging system has resulted in increased productivity for pathologists, concomitant with an expanded role of cytotechnologists in multiple critical steps, including FISH, scan setup, reclassification, and initial interpretation.






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