Primary orbital mantle cell lymphoma: Flow cytometric immunophenotyping as an adjunct to fine-needle aspiration cytology for diagnosis
Sangeeta Verma MD 1, Nalini Gupta MD, DNB 1, Satyawati Mohindra MD 2, Manupdesh Singh Sachdeva MD 3, Arvind Rajwanshi MD 1
1 Department of Cytology and Gynecological Pathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
2 Department of Otolaryngology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
3 Department of Hematology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
|Date of Submission||25-Jun-2016|
|Date of Acceptance||26-Jul-2016|
|Date of Web Publication||20-Jan-2017|
Department of Cytology and Gynecological Pathology, Postgraduate Institute of Medical Education and Research, Chandigarh
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
Verma S, Gupta N, Mohindra S, Sachdeva MS, Rajwanshi A. Primary orbital mantle cell lymphoma: Flow cytometric immunophenotyping as an adjunct to fine-needle aspiration cytology for diagnosis. CytoJournal 2017;14:2
|How to cite this URL:|
Verma S, Gupta N, Mohindra S, Sachdeva MS, Rajwanshi A. Primary orbital mantle cell lymphoma: Flow cytometric immunophenotyping as an adjunct to fine-needle aspiration cytology for diagnosis. CytoJournal [serial online] 2017 [cited 2017 May 27];14:2. Available from: http://www.cytojournal.com/text.asp?2017/14/1/2/198816
Editorial/Peer Review Statement
To ensure the integrity and highest quality of CytoJournal publications, the review process of this manuscript was conducted under a double-blind model (authors are blinded for reviewers and vice versa) through automatic online system.
A 24-year-old male presented with 4 cm × 5 cm-sized polypoidal orbital mass for 8 years, gradually progressive with proptosis of the right eyeball. There was no lymphadenopathy or hepatosplenomegaly. Computed tomography revealed a homogeneous enhancing soft tissue polypoidal mass in the lateral aspect of the right orbit and pushing the eyeball medially. Fine-needle aspiration cytology (FNAC) was performed from the polypoid orbital mass. The diagnosis was offered after cytomorphologic evaluation and flow cytometric immunophenotyping.[Figure 1].
|Figure 1: (a) Computed tomography, axial section showing a homogeneous enhancing soft-tissue swelling in the lateral aspect of the right orbit. A part of eyeball can be seen toward medial aspect; (b and c) Small to medium-sized lymphoid cells with condensed chromatin, inconspicuous nucleoli, and irregular nuclear contours with deep indentations along with many scattered plasma cells (MGG, ×100)|
Click here to view
| » Q1: What Is Your Interpretation?|| |
- Reactive lymphoid proliferation
- Low-grade non-Hodgkin's lymphoma (NHL)
- High-grade NHL
- Hodgkin's lymphoma.
| » Answer|| |
A1: Low-grade NHL [Figure 2].
|Figure 2: Flow cytometric immunophenotyping; (a) CD19-positive events were gated for further analysis; (b) Selected CD19-positive population of cells were CD5-positive and negative for CD23; (c) CD19-positive cells were positive for CD79a; (d) CD19-positive cells were negative for CD10. There was clonal proliferation (with lambda light chain restriction) of B-lineage lymphoid cells (CD19-positive) and these cells were also negative for CD23, CD10, CD3, and kappa light chain|
Click here to view
| » Q2: What Is Your Interpretation After Flow Cytometry?|| |
- Diffuse large B-cell lymphoma
- Chronic lymphocytic lymphoma
- Mantle cell lymphoma
- Hodgkin's lymphoma.
| » Answer|| |
A2: Mantle cell lymphoma.
The correct cytological interpretation is - This is a case of primary mantle cell lymphoma of the orbit.
| » Discussion|| |
Lymphomas primarily involve the axial lymph node groups; although the NHL may also involve extranodal tissues such as nasopharynx, tonsils, gastrointestinal tract, brain, heart, and skin. Ten percent of systemic lymphomas have orbital involvement; however, in <1% cases, lymphoma develops primarily in the orbit. Extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue (MALT)-type is a common type of NHL affecting eye and orbit. Mantle cell lymphoma is a rare entity at this site, which is characterized by the presence of small to medium-sized lymphoid cells with irregular nuclear outlines, often cleaved with condensed chromatin, tiny to absent nucleoli, and scanty slightly basophilic cytoplasm. It is a rare subtype of B-cell NHL occurring in an orbital region with only a few reported cases and case series in literature.,,,,,, Rasmussen et al. identified 21 patients of mantle cell lymphoma in the orbital and adnexal region comprising 9% of all lymphomas in the ocular region. Elderly males were more commonly affected. The orbit (71%) and eyelids (64%) were the most commonly affected sites. Another report  has shown similar results that orbital mantle cell lymphoma has an aggressive clinical course and patients usually present with advanced disease. The present case is a young male with a long history of orbital mass, without any associated systemic findings or peripheral lymphadenopathy. Another case of orbital mantle cell lymphoma has been reported in a 31-year-old female from Nepal, who presenting with orbital swelling of 9-month duration. The various modalities useful for differentiating lymphoproliferative disorders involving orbital region include histopathological examination, immunochemistry, flow cytometry, cytogenetics, and new technologies such as real-time polymerase chain reaction and fluorescence in situ hybridization. The final diagnosis can be given based on combined results of various ancillary techniques. In the present case, FNAC of the orbital mass revealed a population of atypical lymphoid cells having irregular indented nuclei, admixed with plasma cells, histiocytes, and reactive mature lymphocytes. FCI was performed on the aspirated material, which confirmed a clonal population of B lymphoid cells with CD5-positive and CD23-negative cells, the most compatible diagnosis being mantle cell lymphoma. The tumor cells of MALT lymphoma are positive for CD19, CD20, CD79a and negative for CD23; however, they are also negative for CD5, thus differentiating it from mantle cell lymphoma. A definitive diagnosis of mantle cell lymphoma should be offered after demonstration of t(11;14) (q13;q32) translocation or cyclin D1 overexpression, which could not be performed in the present case.
| » Additional Cme Questions|| |
Q3: Which translocation is common in mantle cell lymphoma?
Q4: Mantle cell lymphomas are positive for all of the following, except
- CD 20
- CD 5
- CD 23
- CD 43.
Q5: The best diagnostic marker for mantle cell lymphoma is to demonstrate
- Overexpression of p53
- Beta-catenin expression
- Bcl-6 expression
- Overexpression of cyclin D1.
| » Answers|| |
A3: t(11;14) (q13;q32) translocation.
A4: CD 23; Mantle cell lymphomas are CD23-negative.
A5: Overexpression of cyclin D1.
| » Summary|| |
Primary orbital mantle cell lymphoma is an uncommon neoplasm. It is valuable to subtype NHL as orbital mantle cell lymphoma has relatively poor prognosis as compared to other more common MALT lymphomas of the same region. FNAC combined with flow cytometry gives rapid and accurate results and can be very useful in directing timely management.
| » Competing Interests Statement by All Authors|| |
The authors declare that they have no competing interests.
| » Authorship Statement by All Authors|| |
All authors of this article declare that we qualify for authorship as defined by ICMJE. Each author has participated sufficiently in the work and takes public responsibility for appropriate portions of the content of this article.
| » Ethics Statement by All Authors|| |
As this is a quiz case, this case does not require approval from the Institutional Review Board.
| » List of Abbreviations (In Alphabetic Order)|| |
FCI - Flow cytometric immunophenotyping
FNAC - Fine-needle aspiration cytology
H and E - Hematoxylin and eosin
MALT - Mucosa-associated lymphoid tissue
MGG - May–Grünwald–Giemsa
NHL - Non-Hodgkin's lymphoma.
| » References|| |
Walshe JM, Mulligan AM, O'Keane C, Carney DN. Primary orbital non-Hodgkin's lymphoma – Management and outcome from a single institution. Am Soc Clin Oncol 2002. Abstr. No. 2638
Hon C, Chan RT, Ma ES, Shek TW, Yau K, Au WY. Lymphomatous proptosis as a novel feature of mantle cell lymphoma. Leuk Lymphoma 2006;47:71-5.
Sullivan TJ, Whitehead K, Williamson R, Grimes D, Schlect D, Brown I, et al.
Lymphoproliferative disease of the ocular adnexa: A clinical and pathologic study with statistical analysis of 69 patients. Ophthal Plast Reconstr Surg 2005;21:177-88.
Bairey O, Kremer I, Rakowsky E, Hadar H, Shaklai M. Orbital and adnexal involvement in systemic non-Hodgkin's lymphoma. Cancer 1994;73:2395-9.
Coupland SE, Hummel M, Stein H. Ocular adnexal lymphomas: Five case presentations and a review of the literature. Surv Ophthalmol 2002;47:470-90.
Coupland SE, Krause L, Delecluse HJ, Anagnostopoulos I, Foss HD, Hummel M, et al.
Lymphoproliferative lesions of the ocular adnexa. Analysis of 112 cases. Ophthalmology 1998;105:1430-41.
Looi A, Gascoyne RD, Chhanabhai M, Connors JM, Rootman J, White VA. Mantle cell lymphoma in the ocular adnexal region. Ophthalmology 2005;112:114-9.
Rasmussen P, Sjö LD, Prause JU, Ralfkiaer E, Heegaard S. Mantle cell lymphoma in the orbital and adnexal region. Br J Ophthalmol 2009;93:1047-51.
Kumar R. Mantle cell lymphoma of orbit in a middle aged female – a case report. Nepal Med Coll J 2010;12:275-6.
[Figure 1], [Figure 2]