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 RESEARCH ARTICLE
CytoJournal 2017,  14:5

KRAS detection on archival cytological smears by the novel fully automated polymerase chain reaction-based Idylla mutation test


Department of Public Health, University of Naples Federico II, Naples, Italy

Correspondence Address:
Giancarlo Troncone
Department of Public Health, University of Naples Federico II, Naples
Italy
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1742-6413.200936

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Background: Molecular techniques are relevant to modern cytopathology, but their implementation is difficult without molecular expertise and infrastructure. The assessment of KRAS mutational status on cytological preparations may be useful either to refine uncertain diagnoses on pancreatic aspirates or to yield predictive information to plan targeted treatment of metastatic colorectal cancer (mCRC). The novel test Idylla™ enables fully automated KRAS genotyping in approximately 2 h, even in less experienced hands. Materials and Methods: This study aims to validate this methodology to detect KRAS mutations on archival cytological preparations of pancreatic cancer (n = 9) and mCRC (n = 9) by comparing the Idylla™ performance to that of standard real-time polymerase chain reaction. Results: The same 11 mutations (n = 4: p.G12D; n = 2: p.G12V; n = 2: p.A59E/G/T; n = 1: p.G12R; n = 1: p.G13D; n = 1: p.Q61H) were detected by both techniques. Conclusion: Even in less experienced laboratories, a cytopathologist may easily integrate morphological diagnostic report with accurate KRAS mutation detection, which is relevant for diagnostic and treatment decisions.






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