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Figure 4: Adjusting gain and shutter speed for optimized scan setup. a) The DAPI signal is optimized for the system to detect cell nuclei. The image on the left is similar to what would be seen through the oculars. On the right is a representation of what the system recognizes as nuclei. b) Gain and shutter speeds are adjusted for CEP3 SpectrumRed and CEP 17 SpectrumAqua signals. On the left is an image similar to what would be seen through the microscope; on the right is a summary view of what the system “sees”.

Figure 4: Adjusting gain and shutter speed for optimized scan setup. a) The DAPI signal is optimized for the system to detect cell nuclei. The image on the left is similar to what would be seen through the oculars. On the right is a representation of what the system recognizes as nuclei. b) Gain and shutter speeds are adjusted for CEP3 SpectrumRed and CEP 17 SpectrumAqua signals. On the left is an image similar to what would be seen through the microscope; on the right is a summary view of what the system “sees”.