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Figure 1: (a1 and a2) The pleural fluid smears were very cellular comprising of three-dimensional papillaroid and tubular clusters of malignant cells. The tumor cells showed moderate pleomorphism, clumped chromatin, and prominent nucleoli. In addition, numerous mitotic figures were also noted (Papanicolaou stain). (b) Pleural biopsy showed pleomorphic tumor cells arranged in tubulopapillary and glandular pattern along with fibrocollagenous tissue (hematoxylin and eosin stain). (c1 and c2) Immunohistochemistry showed nuclear positivity for WT1 and nuclear and cytoplasmic staining for Calretinin. (d1) Ascitic fluid smears were highly cellular with formation of cell balls having knobby contours. In higher magnification, features such as cell-in-cell engulfment, giant atypical mesothelial cells were seen. (Papanicolaou stain). (d2) Irregularly distributed variable sized cytoplasmic vacuoles were also seen (May Grunwald Giemsa stain). (e1 and e2) Immunohistochemical stain done on cell block from ascitic fluid sample showing positive staining for calretinin and CK5/6

Figure 1: (a1 and a2) The pleural fluid smears were very cellular comprising of three-dimensional papillaroid and tubular clusters of malignant cells. The tumor cells showed moderate pleomorphism, clumped chromatin, and prominent nucleoli. In addition, numerous mitotic figures were also noted (Papanicolaou stain). (b) Pleural biopsy showed pleomorphic tumor cells arranged in tubulopapillary and glandular pattern along with fibrocollagenous tissue (hematoxylin and eosin stain). (c1 and c2) Immunohistochemistry showed nuclear positivity for WT1 and nuclear and cytoplasmic staining for Calretinin. (d1) Ascitic fluid smears were highly cellular with formation of cell balls having knobby contours. In higher magnification, features such as cell-in-cell engulfment, giant atypical mesothelial cells were seen. (Papanicolaou stain). (d2) Irregularly distributed variable sized cytoplasmic vacuoles were also seen (May Grunwald Giemsa stain). (e1 and e2) Immunohistochemical stain done on cell block from ascitic fluid sample showing positive staining for calretinin and CK5/6